Connexin 43 (pS368)
Cnx43; gap junction 43kDa heart protein

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Product Preparation
A synthetic peptide corresponding to the C-terminal region with a phosphorylated Serine368 of human Connexin 43.
Background
Gap junction proteins allow the intercellular communication among adjacent cells by passing through small molecules less than 1kDa. It is very important in regulating embryonic development, excitable cell contraction, tissue homeostasis, and normal cell growth and differentiation. Gap junctions are composed of integral membrane proteins from the connexin gene family. Approximately 20 members have been cloned and characterized in humans. During intercellular channel formation, six connexin proteins oligomerize into a hemi-channel or connexon followed by connexon trafficking to the plasma membrane. The intact channel is formed when one hemi-channel docks with a second in an opposing cell. Once assembled, groups of these intercellular channels (termed gap junctional plaques) mediate the passage of amino acids, second messengers, and other metabolites between the connected cytoplasmic domains. The channels can be gated in response to various stimuli, including changes in voltage, pH, and connexin phosphorylation. Regulation of gap junctional communication could occur by controlling any one of the steps mentioned above. Phosphorylation of members of the connexin family of gap junction proteins has been correlated with gap junction assembly.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody 43 kDa of Connexin 43 at phosphorylated Serine 368. It reacts with human, rat and mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
2697
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 602-960
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IHC
Reactivity: Hu, Ms, Rt
Price: $ 295.00
REFERENCES
Cooper, C.D. and Lampe, P.D. Casein kinase 1 regulates connesin-43 gap junction assembly. J. Biol. Chem. 277 (47), 44962-44968 (2002).
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