Catenin-beta (pS37)
CTNNB1

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Product Preparation
A synthetic peptide corresponding to the epitope SGIHS with a single phosphorylation site Ser37 of human b-catenin.
Background
Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the ß-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, ß-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3ß and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates ß-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of ß-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3ß activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3ß, allowing unphosphorylated ß-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~88 kDa of human beta-Catenin protein. It also reacts with mouse and rat. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
1499
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 602-600
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 299.00
REFERENCES
Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.
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