Rho(pS188)
Ras homolog gene family, member A variant (RhoA), ARHA, RhoH12
Download Product PDF
MSDS Download Product PDF
Antigen Preparation
A synthetic peptide corresponding to the C-term of epitope KKKSG at a phosphorylation Ser188 of human Rho protein
Background
"Ras homolog gene family consists of RhoA, RhoB, RhoC proteins. These proteins interact directly with protein kinase (Rho kinase) to serve as downstream effectors of the activated GTPase. The Rho proteins are involved in the regulation of the actin cytosekeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family2. Possible mediators for these responses include protein kinase such as p120PKN, and p150, ROKα/Rho-A kinase, myosin light chain (MLC), phosphatase and PIP 5-kinase.3"
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
|
SPECIFICITY
This antibody recognizes ~24 kDa of human RhoA protein. It also reacts with mouse and rat. The other species are not tested
|
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
|
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
|
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|