Desmin (pT76/77)
Desmin, Intermediate filament protein; CSM1, CSM2;

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Product Preparation
A synthetic peptide corresponding to the N-terminus with a dual phosphorylated threonine (Thr76/Thr77) of human Desmin.
Background
Desmin is a major intermediate filament protein found exclusively in striated (skeletal or cardiac) muscle tissue. It belongs to a large family of class III intermediate filament protein, the main intermediate filamentin mature skeletal, cardiac and smooth muscle cells. Mutations in the gene encoding desmin are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. PKC (Protein Kinase C) and cAMP-dependent protein kinase can induce disassembly of the desmin filaments by phosphorylating multiple sites at N-terminus of desmin which can affect the desmin filament structure which play a role in the regulatory process in assembly and turnover.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~55 kDa of human Desmin protein at dual phosphorylated threonine (Thr76/Thr77). It does not recognize non-phosphorylated peptides confirmed by dot blot. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
1674
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 602-280
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 360.00
REFERENCES
Xupei Huang, et al. Protein Kinase C-Mediated Desmin Phosphorylation Is Related to Myofibril Disarray in Cardiomyopathic Hamster Heart. Experimental Biology and Medicine 227:1039-1046 (2002).
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