CDC2 (Paired Y15) (CDK1)
Cyclin-Dependent Kinase 1; CDK1

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Product Preparation
A synthetic peptide surrounding to the epitope -GTYGV- without a phosphorylation site at Tyr15 of human CDC2 protein. This sequence is identical among human, mouse, rat, bovine and chicken species.
Background
Cyclins and cyclin-dependent kinases (CDKs) control cell cycle progression by phosphorlating regulatory proteins. These cyclin-related proteins appear to affect cell structure and function independent of the cell cycle. Cyclins and CDKs have a role in the development and maintenance of cell- and tissue-restricted properties of differentiated cells. CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent kinase 1), is a member of the CDK family of serine/threonin kinases. It is a highly conserved serine protein kinase that plays a key role in regulation of the cell cycle.  The phosphorylation of CDC2 at Y15 and T14 during the G2 phase of the cell cycle inhibits CDC2 activity, while the dephosphorylation of Y15 and T14 by CDC25 phosphatase during the late G2 restores its activity.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes CDC2 (PairedY15) without a phosphorylated site at Tyrosine 15. It does not cross-react with phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
983
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 602-170
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IP
Reactivity: Hu, Ck, Bv
Price: $ 220.00
REFERENCES
Shirwin M. Pockwinse, et al. Cell cycle independent interaction of CDC2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold. Proc Natl Acad Sci U S A. 1997 April 1; 94(7): 3022–3027.
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