CSF-1R (pY723)
Colony stimulating factor 1 receptor; CD115

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Product Preparation
A synthetic peptide corresponding to the epitope –DTYVEM- at a phosphorylation site at tyrosine 723 of human CSF-1R protein. This sequence is identical within human, mouse, rat, and bovine.
Background
CD115, or Colony-stimulating factor-1 receptor (CSF-1R), is the major regulator of macrophage development and associated with epithelial cancers of the breast and ovary. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subquently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. The activated or phosphorylated form of CSF-1R has been associated with enhanced invasive and metastatic potential. The phosphorylation residues includeTyr 708, Tyr 723, Tyr 809 which correlate CSF-1R expression with changes in the growth and development of the cancer stage.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~150 kDa of human CSF-1R protein at a phosphorylation site of Tyrosine 723. It does not recognize non-phosphorylated peptides. This antibody also reacts with mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
1436
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 602-110
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 295.00
REFERENCES
Eugene P. Toy et al. The Activated Macrophage Colony-Stimulating Factor (CSF-1) Receptor as a Predictor of Poor Outcome in Advanced Epithelial Ovarian Carcinoma. (2001) Gynecologic Oncology, 80 (2), 194-200
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