Antigen Preparation
"A synthetic peptide corresponding to the epitope –DTYVEM- at a phosphorylation site at tyrosine 723 of human CSF-1R protein. This sequence is identical within human, mouse, rat, and bovine."
Background
"CD115, or Colony-stimulating factor-1 receptor (CSF-1R), is the major regulator of macrophage development and associated with epithelial cancers of the breast and ovary. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subquently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. The activated or phosphorylated form of CSF-1R has been associated with enhanced invasive and metastatic potential. The phosphorylation residues includeTyr 708, Tyr 723, Tyr 809 which correlate CSF-1R expression with changes in the growth and development of the cancer stage."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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