Catenin-beta (pY96)
CTNNB1

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Product Preparation
A synthetic peptide corresponding to the epitope LLYST at the phosphorylation site Tyr96 of human b-catenin.
Background
Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the ß-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, ß-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3ß and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates ß-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of ß-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3ß activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3ß, allowing unphosphorylated ß-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~88 kDa of human beta-Catenin protein. It also reacts with mouse and rat. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
1499
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 602-020
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: ELISA, WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 299.00
REFERENCES
Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.
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