CDC25b (Paired S42)
Cell Division cycle 25b

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Product Preparation
A synthetic peptide surrounding of –LGSPV- of human CDC25B protein WITHOUTthe phosphorylation site at Serine 42.
Background
Three homologues of CDC25 called CDC25A/B/C Cdc25 tyrosine phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes CDK1 (CDC2). Dephosphorylation of threonine 14 and tyrosine 15 of CDK1 by CDC25 phosphatases is a key step in the activation of the CDK1-cycline B protein kinase. Of the three mammalian Cdc25 isoforms, Cdc25A is phosphorylated by genotoxic stress-activated Chk1 or Chk2, which triggers its SCFbeta-TrCP-mediated degradation. CDC25B is degraded by the proteasome. CDK1-cyclin A phosphorylation targets CDC25B for degradation and this might be an important component of cell cycle regulation at the G2/M transition.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes NON-phosphorylation site (S42) of human CDC25B protein. It does not recognize the phosphorylated CDC25B at Serine 42.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
994
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 601-620
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Elisa, WB, IHC
Reactivity: Hu
Price: $ 295.00
REFERENCES
Véronique Baldin, et al, Phosphorylation of Human CDC25B Phosphatase by CDK1-Cyclin A Triggers Its Proteasome-dependent Degradation. The Journal of Biological Chemistry, 272, 32731-32734 (1997).
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