CDC25b(pS42)
Cell Division cycle 25b

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Product Preparation
A synthetic peptide surrounding of –LGSPV- of human CDC25B protein with a phosphorylation site Serine 42.
Background
Three homologues of CDC25 called CDC25A/B/C Cdc25 tyrosine phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes CDK1 (CDC2). Dephosphorylation of threonine 14 and tyrosine 15 of CDK1 by CDC25 phosphatases is a key step in the activation of the CDK1-cycline B protein kinase. Of the three mammalian Cdc25 isoforms, Cdc25A is phosphorylated by genotoxic stress-activated Chk1 or Chk2, which triggers its SCFbeta-TrCP-mediated degradation. CDC25B is degraded by the proteasome. CDK1-cyclin A phosphorylation targets CDC25B for degradation and this might be an important component of cell cycle regulation at the G2/M transition.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes phosphorylated Serine 42 of human CDC25B protein. It does not recognize the nonphosphorylated CDC25B.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
994
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 601-610
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Elisa, WB, IHC
Reactivity: Hu
Price: $ 375.00
REFERENCES
Véronique Baldin, et al, Phosphorylation of Human CDC25B Phosphatase by CDK1-Cyclin A Triggers Its Proteasome-dependent Degradation. The Journal of Biological Chemistry, 272, 32731-32734 (1997).
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