Insulin receptor substrate 1; IRS1

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Product Preparation
A synthetic peptide corresponding to the epitope DGYMP with a single phosphorylation site Tyr612 of human IRS-1.
Insulin Receptor Substrate-1 (IRS-1), 165 kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser312 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Tyr612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKC, resulting in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
This antibody recognizes ~165 kDa of human IRS-1 (pTyr 612). It also reacts with mouse and rat. The other species are not tested.
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
0.01-0.1 µg/ml
2-5 µg/ml
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 601-600
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: Elisa, WB, IHC
Reactivity: Hu, Rt, Ms, Ck, canis,
Price: $ 345.00
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304–45307.
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