S6K(pS434)
ribosomal protein S6 kinase; S6K; p70-S6K

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Product Preparation
A synthetic peptide corresponding to the epitope –IRSPR- at a phosphorylation site at serine 434 of human p70S6K protein. This sequence is identical within human, rat, mouse, bovine, chicken and dog.
Background
S6 kinase, or p70 S6 Kinase, a mitogen activated Ser/Thr protein kinase, is required for cell growth and G1 cell cycle progression. P70 S6 Kinase and single 4E-BP are phosphorylated via the insulin and target of rapamycin (TOR) signaling pathway which is independent of PI3K and AKT activity. On the other hand, the oncogenic transformation by P3k and Akt is dependent on targets that control translation: transforming activity , which is correlated with phosphorylation and activation of S6K and eliminated by the mTOR inhibitor rapamycin. S6K phosphorylates the S6 protein of the 40S ribosomal subunit.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~70 kDa of human p70S6K protein. It does not recognize non-phosphorylated peptides. This antibody also reacts with mouse and rat. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
6198
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 601-030
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB
Reactivity: Hu, Ms, Rt
Price: $ 215.00
REFERENCES
Mathieu Miron, et al. Signaling from Akt to FRAP/TOR Targets both 4E-BP and S6K in Drosophila melanogaster. (2003) Molecular and Cellular Biology, 23 (24), 9117-9126.
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