Casein (kappa)
CSN3, CSN10, CSNK, Casein Kappa

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Product Preparation
A synthetic peptide corresponding to the N-terminus of mouse Casein protein.
Background
Casein is the predominant phosphoprotein which has no disulphide bridges but rich in proline. It is found in fresh milk, as a salt of calcium. Casein is not coagulated by heat. It is precipitated by acids and by rennet, a proteolytic enzyme typically obtained from the stomachs of calves. The enzyme trypsin can hydrolyze off a phosphate-containing peptone. This protein is synthesized by mammary epithelium only in the presence of lactogenic hormone combination of insulin, hydrocortisone, and prolactin. Enhancement of the rate of secretion of newly-synthesised caseins occurs when mammary explants are challenged in vitro with agents that activate protein kinase A (PKA). The PKA-sensitive step(s) in casein secretion is early in the exocytosis pathway but inhibition of PKA does not impair casein maturation. PKA is located on membranes of vesicles situated in the Golgi region. Casein positive cells are present in all breast carcinomas with a variable distribution and degree of staining. Casein serves as an in vitro marker of terminally differentiated breast luminal epithelial cells.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~21 kDa of mouse Casein protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
1448
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-830
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA
Reactivity: Ms
Price: $ 220.00
REFERENCES
P. Chandra Shekar et al. κ-Casein-deficient mice fail to lactate. PNAS 2006 May 23, 103 (21): 8000-8005.
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