Aggrecan/AGC1
Aggrecan

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Product Preparation
A synthetic peptide corresponding to the 380-400 aa of human ACAN protein. This sequence is identical to bovine, mouse and rat.
Background
Aggrecan or aggregating chondroitin sulfate proteoglycan, is an integral part of the extracellular matrix in cartilagenous tissue. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight. The cleavage of Aggrecan has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~250 kDa of human Aggrecan protein. It reacts with mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
176
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-670
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA
Reactivity: Hu, Ms, Rt
Price: $ 215.00
REFERENCES
Sandy,J.D., et al. The structure of aggrecan fragments in human synovial fluid. Evidence for the involvement in osteoarthritis of a novel proteinase which cleaves the Glu 373-Ala 374 bond of the interglobular domain. J. Clin. Invest. 89 (5), 1512-1516 (1992)
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