Antigen Preparation
"A synthetic peptide corresponding to the N-terminus of Calumenin protein. This sequence is identical to rat, mouse and human origins."
Background
"Calumenin (CALU) is a calcium binding protein localized in the endo/sarcoplasmic reticulum (ER) in mammalian tissues. It is involved ER and Golgi apparatus named CERC which is an acronym for its family members Cab-45, reticulocalbin, Erc-55 and Calumenin. The CALU gene encodes a deduced 315aa protein containing six- EF-hand Ca2+-binding protein with one potential N-glycosylation site, and a C-terminal ER retention signal. The human and mouse/rat CALU proteins are 98% identical. Two isoforms exist in cardiac and skeletal muscles, but Calumenin-2 is the main isoforms in skeletal muscle. Increasing in the expression of Calumenin in chemotherapeutic drug resistance in neoplastic cells is observed. It is also involved in the pathophysiology of thrombosis or in wound healing. CALU mRNA is ubiquitously expressed in human tissues and maps to 7q32."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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