Antigen Preparation
"A synthetic peptide of PY EYL RPS VVE NSV AI corresponding to the internal portion of human LOX1 protein sequence. It is identical among human, rat and mice."
Background
"Lipoxygenases constitute a family of enzymes which are implicated in a variety of inflammatory disorders including asthma. The 15-lipoxygenase has been identified as the major route of arachidonic acid metabolism in human lung, airway epithelial cells, eosinophils, and developing red cells, the localization of the enzyme within lung has not been clearly defined. The different isoforms of 15-lipoxygenase exist in different tissues in basal and ciliated cells of the trachea, bronchi and bronchioles of lungs. The 15-lipoxygenase of human lung is identical to that of the reticulocyte enzyme and is preferentially expressed in airway epithelium."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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