Interleukin-1 receptor-associated kinase 4; IRAK4

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Product Preparation
A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein. This sequence is identical among human, mouse, bovine and dog.
IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK family member necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex.
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
This antibody recognizes IRAK-4(pT345) with a phosphorylated site at Threonine 345. It does not cross-react with non-phosphospecific peptide.
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
0.01-0.1 µg/ml
2-5 µg/ml
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-560
Lot #: See the label
Size: 100 ug
Host: Mouse
Isotyping: Mouse IgG
Applications: WB, ELISA
Reactivity: Hu, Ms, Bv, Dg
Price: $ 230.00
Li S et al (2002) IRAK-4, A novel member of the IRAK family with the properties of an IRAK-kinase. PNAS 99 (8): 5567-5572.
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