CD146
Melanoma cell adhesion molecule (MCAM); MUC18; CD1

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Product Preparation
Recombinant human CD146 protein encoding aa 433-647 expressed in E.coli.
Background
CD146 belongs to the immunoglobulin super-gene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody reacts with human CD146. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
4162
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-11134
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC-P
Reactivity: Hu
Price: $ 349.00
REFERENCES
Johnson,J.P., Rothbacher,U. and Sers,C. The progression associated antigen MUC18: a unique member of the immunoglobulin supergene family. Melanoma Res. 3 (5), 337-340 (1993).
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