JNK/SAPK1(pT183/pY185)
Jun N-terminal kinase; MAPK8

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Product Preparation
A synthetic peptide surrounding to the epitope -TPY- with dual phosphorylation sites at Thr183 and Tyr185 of human JNK/SAPK .
Background
c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK). There are three isoforms, JNK1 (~49 kDa), JNK2 (~55 kDa), and JNK3 (~54 kDa). JNK is dually phosphorylated at Thr 183 and Tyr 185 by protein kinases MKK4 and MKK7. The JNK and p38 pathways function to modulate cell-cycle, apoptotic and transcriptional responses to stress.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes human JNK protein. It also reacts with rat and mouse JNK protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
5599
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-380
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA, IP
Reactivity: Hu, Rt
Price: $ 340.00
REFERENCES
Yogev,O., et al. DNA damage-dependent translocation of B23 and p19 ARF is regulated by the Jun N-terminal kinase pathway. Cancer Res. 68 (5), 1398-1406 (2008)
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