GSK3a(pS21)
Glycogen Synthase Kinase 3a; GSK3A;
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Antigen Preparation
"A synthetic peptide surrounding to the epitope -RTSSFA- with a phosphorylation site at Ser21 of human GSK3-alpha protein. This sequence is identical among human, mouse and rat."
Background
"Glycogen Synthase Kinase-3 has two isoforms, GSK-3α and GSK3β. GSK3 is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase as well as the regulation in Wnt signal pathway. GSK-3 alpha reduces the substrates beta-catenin and tau to imply an important role in Wnt signaling and tauopathies such as Alzheimer’s disease.."
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
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SPECIFICITY
This antibody recognizes GSK-3 alpha (pS21) with a phosphorylated site at Serine 9. It does not cross-react with non-phosphospecific peptide.
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FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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