FAK(pY576)
Focal adhesion kinase

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Product Preparation
A synthetic peptide surrounding to the epitope -STYYKA- with a phosphorylation site at Tyrosine 576 of human FAK protein. This sequence is identical among human, mouse and rat.
Background
Focal adhesion kinase (FAK) gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinase but lacks significant sequence similarity to kinase from other subfamilies. Activation by phosphorylation of Y397, Y576/577 and Y863 of FAK protein may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes FAK (pY576) with a phosphorylated site at Tyrosine 576. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
25614
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-320
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA, IP
Reactivity: Hu
Price: $ 230.00
REFERENCES
Leu TH, et al. Tyr-863 phosphorylation enhances focal adhesion kinase autophosphorylation at Tyr-397. Oncogene. 2002 Oct 10;21(46):6992-7000.
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