AKT2/PKBb
v-akt murine thymoma viral oncogene homolog 2; PKB

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Product Preparation
A synthetic peptide corresponding to the epitope 455-469 aa of human AKT2 protein.
Background
Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (a), Akt2 (ß) and Akt3 (?)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473 (S473). Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody only recognizes ~60 kDa of human AKT2, not AKT1 or AKT3. It also reacts with mouse and rat AKT2 protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
208
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-230
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA, IP
Reactivity: Hu, Ms, Rt
Price: $ 199.00
REFERENCES
Mei Sun et al. Phosphatidylinositol-3-OH Kinase (PI3K)/AKT2, Activated in Breast Cancer, Regulates and Is Induced by Estrogen Receptor _ (ER_) via Interaction between ER_ and PI3K1. CANCER RESEARCH 61, 5985–5991, August 15, 2001.
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