AKT2/PKBb
v-akt murine thymoma viral oncogene homolog 2; PKBB, PKBBETA, PRKBB, RAC-BETA
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Antigen Preparation
A synthetic peptide corresponding to the epitope 455-469 aa of human AKT2 protein.
Background
"Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (α), Akt2 (β) and Akt3 (γ)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473 (S473). Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody only recognizes ~60 kDa of human AKT2, not AKT1 or AKT3. It also reacts with mouse and rat AKT2 protein. The other species are not tested. "
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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