AKT1/PKBa(pS473)
v-akt murine thymoma viral oncogene homolog 1; AKT

PDF Image Download Product PDF
Product Preparation
A synthetic peptide containing the phosphorylated Ser473 motif of human AKT1.
Background
Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (a), Akt2 (ß) and Akt3 (?)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473 (S473). Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
The motif corresponding to the major autophosphorylation site of the human AKT1 kinase protein. This antibody does not cross react to the non-phosphorylated AKT1 or with other unrelated phosphorylated serine. Ser473 corresponds to QFSYS in human, rat, mouse, chicken and canis of AKT/PKB proteins
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
207
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-220
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, ELISA, IP
Reactivity: Hu, Ms, Rt
Price: $ 220.00
REFERENCES
N/A
©2011-2015 Abbomax.com. All rights reserved.