AKT1/PKBa
v-akt murine thymoma viral oncogene homolog 1; AKT

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Product Preparation
A synthetic peptide corresponding to the close to C-term of human AKT1 protein.
Background
Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (a), Akt2 (ß) and Akt3 (?)]; Akt2 and Akt3 have 81% and 83% homology in amino acid sequences with Akt1, respectively. Numerous studies have revealed that the expression pattern of Akt1 and Akt2 is ubiquitous, whereas Akt3 is expressed predominantly in brain, heart, and kidney. Akt is activated by insulin and growth and survival factors .Akt has been referred to as an oncogene because it has increased activity in a number of tumors.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody only recognizes ~60 kDa of human AKT1, not AKT1 or AKT2. It also reacts with mouse and rat AKT3 protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
207
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 600-200
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 199.00
REFERENCES
Kaname Nakatani, et al. Up-regulation of Akt3 in Estrogen Receptor-deficient Breast Cancers and Androgen-independent Prostate Cancer Lines. THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 274, No. 31, Issue of July 30, pp. 21528–21532, 1999.
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