PKC (pS345)
Metastasis-associated gene family, member 2 (MTA2)

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Product Preparation
A synthetic peptide corresponding to the epitope -EDR-S-KSAP- with a phosphorylated Ser 345 of human PKC.
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. With cytotoxic dose (100 uM) of anticancer drug, cisdiamminedlchloroplatinum(ll) (CDDP) incubation, the phosphorylation and translocation into nuclear of PKC in cancer cells including SKBR-3 which express different PKC isoforms was found. Overexpression of PKC was reported in several hemopoietic cell lines and tumors. PKC-epsilon was considered as a full oncogenic potential.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody only recognizes PKC phosphorylated at Serine 345, but not the non-phosphorylated 345. It is human, rat and mouse reactive.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
9219
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-9244
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 250.00
REFERENCES
T Ohmori and CL Arteaga. Protein kinase C epsilon translocation and phosphorylation by cis-diamminedichloroplatinum(II) (CDDP): potential role in CDDP-mediated cytotoxicity. Cell Growth & Differentiation, 9 (4): 345-353 (1998).
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