NFkBp65 (pS276)
nuclear factor kappa B subunit p65; NFkB

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Product Preparation
A synthetic peptide derived from epitope –LRRPSDRE- with the phosphorylation site Ser276 of human NF-kB p65 protein. This sequence is identical to human, mouse and rat.
Background
NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50/NFkB1)) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the proto-oncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKK beta .
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes NFkB p65 with the phosphorylation site Ser276. It does not cross-react with non-phosphospecific peptide.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
5970
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-9064
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, IHC, ELISA, IP
Reactivity: Hu/Rt/Ms
Price: $ 285.00
REFERENCES
Cheshire,J.L. and Baldwin,A.S. Jr.Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation. Mol. Cell. Biol. 17 (11), 6746-6754 (1997).
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