IRS-2 (pS731)
Insulin receptor substrate 2; IRS2

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Product Preparation
A synthetic peptide corresponding to the epitope ASSPA with a single phosphorylation site Ser 731 of human IRS-2.
Background
Insulin Receptor Substrate-2 (IRS-2), a 180kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, and IRS 4. IRS 2, a signaling molecule that mediates effects of insulin, Insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP-dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has a dual function, serving either for a positive or negative modulation of insulin signal transduction.
PURIFICATION
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
SPECIFICITY
This antibody recognizes ~180 kDa of human IRS-2 (pSer731). It also reacts with mouse and rat. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
8660
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-9004
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB, IP
Reactivity: Hu/Rt/Ms
Price: $ 290.00
REFERENCES
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304–45307.
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