Biotin
Biotin

PDF Image Download Product PDF
Product Preparation
Biotin-conjugates as an immunogen.
Background
This antibody has Ab-1 has a very high affinity for biotin and is excellent for use in various amplification techniques. It recognizes both the free and protein conjugated (either soluble or cell bound) form of biotin. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
This antibody recognizes free Biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
N/A
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-7124
Lot #: See the label
Size: 100 ug
Host: Mouse
Isotyping: Mouse IgG1
Applications: WB, FC
Reactivity: all species
Price: $ 199.00
REFERENCES
Elisabeth D. Coene et al (2008). Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin. Methods in Molecular Biology, Volume 418. 157-170.
©2011-2015 Abbomax.com. All rights reserved.