BCL-2
B-cell CLL/lymphoma 2

PDF Image Download Product PDF
Product Preparation
A synthetic peptide derived from C-terminus of human BCL-2 protein. This sequence is identical in human, rat, mouse, bovine and dog.
Background
The Bcl-2 gene codes for a 25 kDa protein which has been implicated in the control of cell death by apoptosis, in normal tissues, expression of bcl-2 is restricted to cells and tissues that are characterized by apoptotic cell turnover. Bcl-2 protein exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release, thus it is also considered as an inner mitochondrial membrane protein which can be expressed in lymphoma and breast carcinoma cells. This protein modulates mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, Ser87 and Thr127 which may be the targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Phosphorylation of Bcl-2 at Ser70 by IL-3/JNK may be required for its enhanced antiapoptotic functions.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes Bcl-2 protein. It cross reacts to human, rat and mouse, The other species not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
596
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-4734
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB
Reactivity: Hu
Price: $ 299.00
REFERENCES
Camlica H., et al. Statistical interpretation of CA125 and Bcl-2 in serum of patients with late stage ovarian cancer. Am J Clin Oncol, 2008 Dec, 31(6): 585-8.
©2011-2015 Abbomax.com. All rights reserved.