Antigen Preparation
A synthetic peptide derived from C-terminus of SARS-COV-2 N (nucleocapsid) protein
Background
"The SARS-CoV-2 nucleocapsid (N) is a 45 kDa protein, one of the major structural proteins of the virus. It binds directly to viral RNA and provides stability. N-protein is involved in the transcription and viral RNA replication, packaging of the encapsidated genome into helical ribonucleocapsid, and interference with cell cycle processes of host cells. This most abundant protein of coronavirus is composed of two structural domains and a linker region. N-protein contains two independent RNA binding domains, the N-terminal RNA binding domain and a C-terminal domain, which can interact with the viral RNA to form the ribonucleoprotein. The linker region binds to the M protein. The N-protein is a highly immunogenic phosphoprotein and is extremely conserved. This protein is a repressor of RNA interference and an antagonist of interferon. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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