Antigen Preparation
Recombinant protein encoding aa 1119-1304 of human CD45.
Background
"CD45 leucocyte common antigen (LCA) belongs to the family of at least four isoforms of membrane
glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic
cell lines, normal and malignant non-hematopoietic tissues. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The intracellular portion of these molecules has protein phosphatase activity and is involved in regulation of transmembrane signals. CD45 plays an essential role in lymphocyte activation.
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Applications/Suggested Working Dilutions
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Flow cytometry
Not tested
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