CD14
lipopolysaccharide (LPS) receptor; CD14

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Product Preparation
Recombinant protein encoding aa 1-201 of human CD14
Background
CD14, also known lipopolysaccharide (LPS) receptor, is a 55 kDa glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein. It is expressed at high levels on monocytes and macrophage, and at lower levels on the surface of granulocytes and neutrophils. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. CD14 is anchored to cells by linkage to GPI and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 is involved in the clearance of gram-negative pathogens and in the up-regulation of adhesion molecules and cytokines expression in monocytes and neutrophils.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody reacts with human CD14. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
929
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
N/A
Immunoprecipitation
N/A
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 500-10554
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: WB
Reactivity: Hu
Price: $ 349.00
REFERENCES
1. SC Stocks, MA Kerr, C Haslett, and I Dransfield . CD66-dependent neutrophil activation: a possible mechanism for vascular selectin-mediated regulation of neutrophil adhesion. J. Leukoc. Biol., Jul 1995; 58: 40. SD Wright, RA Ramos, PS Tobias, RJ Ulevitch, and JC Mathison. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 21 September 1990 249: 1431-1433.
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