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CD3 zeta

CD247; CD3-zeta;

Product Preparation

A synthetic peptide corresponding to the N-terminus of human CD3 zeta. This sequence is identical among human, rat and mouse species.

Background

CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). It forms three dimmers: a heterodimer of gamma and epsilon chain, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. The CD3 zeta plays an important role in coupling antigen recognition to several intracellular signal transduction pathway. Low expression of the antigen results in impaired immune response.

PURIFICATION The Rabbit IgG is purified by Epitope Affinity Purification	SPECIFICITY This antibody recognizes human CD3 zeta protein. It cross-reacts to human, mouse and rat. The other species was not tested.
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.	STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

919

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

5-10 µg/ml

Order Info

Catalog #:	500-3054
Lot #:	See the label
Size:	100 ug
Host:	Rabbit
Isotyping:	Rabbit IgG
Applications:	IHC(P), WB, IP
Reactivity:	Hu, Ms, Rt
Price:	$ 299.00

REFERENCES

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfidelinked) CD3delta proteins in murine T lymphocytes. J Biol Chem. (1998) 273(23):141527.