14-3-3 beta
YWHAB; tyrosine 3-monooxygenase/tryptophan 5-monoo

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Product Preparation
A synthetic peptide corresponding to N-term of human 14.3.3 beta protein.
Background
The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~28 kDa of human 14.3.3 beta. It does not react to the other isoforms. This antibody reacts with human, rat and mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
7529
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-2924
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB, IP
Reactivity: Hu, Ms, Rt
Price: $ 189.00
REFERENCES
Zhi-jun Luo, et al. Identification of the 14.3.3 Domains Important for Self-association and Raf Binding. J. Bio. Chem 270 (40), 23681–23687, 1995
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