Caspase 9
CASP9

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Product Preparation
A synthetic peptide derived from N-terminus of human Caspase 9 protein
Background
Caspase-9 belongs to a member of the cysteine-aspartic acid protease (Caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small. Those dimerize to form the active enzyme. Activation of procaspase-9 by Apaf-1 in the Cytochrome C/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioner caspase-3, -6 and -7.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~47 kDa of human Caspase 9. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
842
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-2294
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB, IP
Reactivity: Hu
Price: $ 399.00
REFERENCES
Duan, H et al. ICE-LAP6, a novel member of the ICE/CED-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271 (28), 16720-16724(1996).
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