Caspase 7
Caspase-7; CASP7; ICE-IAP3; CMH-1; MCH3

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Product Preparation
A synthetic peptide derived from N-terminus of human Caspase 7 protein
Background
Caspase-7 belongs to a member of the cysteine-aspartic acid protease (Caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small. Those dimerize to form the active enzyme. The precursor of this caspase is cleaved by Caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Caspase-7(CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis. It is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B . Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes ~35 kDa of human Caspase 7. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
840
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-2274
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: Hu
Price: $ 399.00
REFERENCES
Lippke, J. A et al. Identification and characterization of CPP32/MCH2 homolog 1, a novel cysteine protease similar to CPP32. J. Biol. Chem. 271 (4), 1825-1828 (1996).
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