CD3
CD3

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Product Preparation
A synthetic peptide corresponding to extracellular domain of human CD3.
Background
CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. This anti-CD3 antibody is commonly used as a phenotypic marker for human T cells.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes CD3 protein. This antibody reacts with human, rat and mouse. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
919
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-1254
Lot #: See the label
Size: 100 ug
Host: Rabbit
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: Hu, Mk, Dg
Price: $ 329.00
REFERENCES
Kearse KP. Calnexin associates with monomeric and oligomeric (disulfidelinked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):141527.
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