c-erbB-2/HER-2
c-erbB2/neu protein

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Product Preparation
A synthetic peptide corresponding to the C-terminus of Her2 protein. This sequence is identical in human, mouse, and rat.
Background
Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
This antibody recognizes Her-2 protein. It cross-reacts with human, rat and mouse. The other species was not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
2064
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
5-10 µg/ml
Order Info
Catalog #: 500-12424
Lot #: See the label
Size: 100 ug
Host: Mouse
Isotyping: Mouse IgG1
Applications: IHC-P, WB
Reactivity: Hu, Ms, Rt
Price: $ 255.00
REFERENCES
Xiao-Feng Le et al (2000) Anti-HER2 Antibody and Heregulin Suppress Growth of HER2-Overexpressing Human Breast Cancer Cells through Different Mechanisms. Clinical Cancer Research Vol. 6, 260-270.
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